I've used it for mammalian cells, hepatic, ovarian, intestinal and it works correctly

Thanks, but please could you provide me a reliable protocol?

Dear Pier Paolo Danielli

Please find the attached reference document for Phototoxicity assay (OECD guideline)

Article The 3T3 neutral red uptake phototoxicity test: Practical exp...

CC50 LD50 correlation with Human keratinocytes

Keratinocyte NRU protocol

Thanks Sundarajan.

Hi,

In our studies toxicity evaluations were performed using HBE cells and two cytotoxicity assays instead of just one. This is because certain chemicals have been reported to give divergent results in different toxicity tests including the NRU and MTT assays (Olivier et al 1995; Chiba et al 1998). Besides, Evans et al (2001) have recently found that in some cases one of the NRU or MTT assays can be more sensitive in detecting the toxicity of non-viral transfection reagents.

*****

Evans, A.R., Alexander, D., Burke, P. and Reed, C.J. (2001) Toxicity and transfection efficiency: comparison between four commercially available non-viral transfection reagents in a human bronchial epithelial cell line. British Pharmaceutical Conference Science Proceedings 2001,  106.

******* 

Evans, A. (2003) In Vitro and Ex Vivo Studies on the Toxicity and Efficacy of a Selection of Non-Viral Transfection Reagents.  PhD Thesis, Liverpool John Moores University, Liverpool, UK.

*********

Please also see attached scientific paper.