I need to know how to use this reagent with my membranes.
I have been trying to use the reverse line blotting to detect several tick borne pathogens. However, I get very large signals. I have decreased the concentration of both PCR products and the...
09 October 2014 8,364 8 View
I am using nested PCR to diagnose Candidatus neoehrlichia mikurensis 16S rRNA gene and I got bright bands at the exact size I am seeking after the nested PCR and electrophoresis. I extracted the...
09 October 2012 8,394 4 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....
28 July 2024 9,545 6 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...
18 July 2024 7,258 3 View
Dear All, I would like to ask about the relation between the surface energy and solidification of polymer. If we apply a chemical treatment such as NaOh solution or silane treatment on the...
17 July 2024 190 3 View