Mohamed Abdallah Mohamed Moustafa

3 Questions 10 Answers 0 Followers

Questions related from Mohamed Abdallah Mohamed Moustafa

Has anyone used Streptavidin−Peroxidase Polymer, Ultrasensitive from Sigma Aldrich for RLB hybridization?

I need to know how to use this reagent with my membranes.

09 September 2015 7,969 0 View

Why do the Reverse Line Blotting result signals appear large and overlapping with the adjacent ones?

I have been trying to use the reverse line blotting to detect several tick borne pathogens. However, I get very large signals. I have decreased the concentration of both PCR products and the...

10 October 2014 8,334 8 View

Candidatus Neoehrlichia mikurensis, The sequencing is not detecting the pathogen even when I used the bright bands from the gel for sequencing!

I am using nested PCR to diagnose Candidatus neoehrlichia mikurensis 16S rRNA gene and I got bright bands at the exact size I am seeking after the nested PCR and electrophoresis. I extracted the...

10 October 2012 8,376 4 View