Shweta Johari

8 Questions 5 Answers 0 Followers

Questions related from Shweta Johari

What is the best way to represent KEGG pathways?

Hello, I have multiple pathways that are being studied due to single-cell sequencing. What is the best way to represent pathways or specifically KEGG pathways for a manuscript? Other than...

05 June 2023 3,959 3 View

What is the best way of representing IHC data?

Good day! I have used QuPath as the software for analyzing IHC data and then exported the excel sheet of measurement of annotation with the following columns. Image, Name, Class Parent, ROI,...

01 March 2023 6,177 0 View

Is there another alternative to understand DGE instead of QIAGEN Ingenuity Pathway Analysis?

Hello, I am interested if there is another software that can give us holistic knowledge of differential gene expression (DGE) like how IPA produces. IPA is licensed, Is there another software...

17 January 2023 6,419 1 View

What method of analysis are you using for Cell Plex data?

Hello, Is anyone working on Cell Plex using 10X Genomics reagents? My question is related to the method of analysis you are using for the analysis of single-cell sequencing using Cell...

26 May 2022 6,175 4 View

Can you please recommend a Training Course for Single- Cell Analysis?

Good day! Does anyone know any particular training course that has helped you in single-cell analysis? If anyone can please recommend ways to gain more knowledge in Cell Ranger, Loupe Browser, or...

22 October 2021 2,640 2 View

Has the antibody concentration ever fluctuated 10 times with your IHC?

For months, I have used F4/80 antibody on zinc fixed slides with a concentration of 1:1200, suddenly for the past two weeks, the 1:1200 dilution was not working and after reoptimizing it only...

03 August 2021 8,283 3 View

Has anyone used PEN membrane glass slides (Arcturus, LCM0522) and stained it with Cresyl Violet Solution?

I have an unstained section in the PEN membrane slide already and need to stain it with Cresyl Violet Stain to isolate RNA/DNA, do I directly put the solution on the section or do I dehydrate...

07 January 2021 715 0 View

Phenotyping Mouse Tissues on inform?

Good evening Has anyone done phenotyping on mouse tissue using inform software, Can someone tell me the membrane threshold and cytoplasm threshold and size of the nucleus? any idea! Thank you...

14 September 2020 4,546 0 View