I was wondering if anyone has tried to induce EAE in old mice (above 18m) and wether the score is more or less severe in aged mice.
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!
25 February 2021 3,092 2 View
Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...
02 February 2021 3,455 4 View
Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?
23 December 2020 5,058 7 View
Hello, everybody. I would like to apply machine learning techniques to clinical data of patients with Covid19. However, I can not find any clinical database of Covid patients, do you know any...
02 December 2020 706 8 View
I have 68nm spherical Au NPs, which gave me a concentration of 1.22 x 10^9 particles/mL. How can I calculate the molarity without the need for TEM?
22 November 2020 8,850 3 View
In the research that I am working on, I need to migrate a graph database to sql to carry out tests, I am using neo4j as a tool, the information I have found is about migrating from SQL to neo4j...
09 November 2020 1,869 3 View
I want to calculate grain size and microstrains. I fitted my peaks with a Voigt function but the FWHM of the Loretzian contribution resulted to be nearly zero, lower than the FWHM obtained from...
28 October 2020 2,578 1 View
Hi. I'm working with soil enzymes and I have an issue because i saw several papers that used "integrated biological response" or IBRv2 to integrate data from enzymes and build a hexagonal star...
11 October 2020 5,131 3 View
In view of the potential for Covid-19 reinfection and the weak immune response and short duration in patients previously infected with Covid-19, many doubts have emerged if the virus is capable of...
03 October 2020 5,689 7 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View