I am trying to develop a method for the determination of acrylamide using HPLC coupled to Orbitrap mass spectrometer. The problem is that there is a kind of conatmination by fragments ions (m/z 55 for acrylamide and 58 for deuterated acrylamide) . The fragment ions appear in the chromatogram in a 5 min window, making quantification extremely difficult.
Why is this hapenning in orbitrap? Only the quadrupole may be used for quantification of acrylamide?
Mr. Guido,
Please pay attention to this paper:
Rapid Commun Mass Spectrom. 2012, 26, 499-509.
Comparison between a high-resolution single-stage Orbitrap and a triple quadrupole mass spectrometer for quantitative analyses of drugs.
Henry H, Sobhi HR, Scheibner O, Bromirski M, Nimkar SB, Rochat B.
There are derivatization methods. Perhaps xanthydrol. There has been GC work done on it but it might be useful for LC/MS as well. That way you will get higher more unique mass fragments.
Thanks for answering.
Steven, I know the GC/MS method by using xanthydrol. However, I prefer to use the high resolution Orbitrap mass spectrometer for quantitative analysis of acrylamide.
Bojidarka, your recommended paper has been very useful. This is a very interesting paper comparing the orbitrap exactive-MS with triple quadrupole MS. I found that the exact masses of full scan HR may be used to distinguish the acrylamide and deuterated acrylamide, with no need for fragmentation. Specially, the resolution is higher for lower m/z, which is the case for acrylamide.
Any contribute regarding these topics is appreciated.
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