Has anyone tried extracting RNA from dead tissue or cells? Would one expect to get no RNA at all as it's definitely degraded or should you expect to get RNA by nanodrop but just the quality would be terrible? I tried extracting RNA from dead skin from patients and am getting no detectable RNA by nanodrop. I don't think it's a technical issue as I've tried multiple kits and still get 0 or negative RNA values. What is confounding is that I was expecting to get some RNA but expected the quality to be bad as opposed to getting nothing.
Hi Pranali
the issue in extracting RNA or DNA from tissues is how these tissues have been stored. If they are fresh no problem at all, but for old tissues degradation will give less success in RNA preparation. there are some protocols to keep tissue the most away from degradation, but the most easy and cheap is to get them at -80°c (or deeper...) in alcool. lot of papers in punned, you'll find.
Not surprised to get no PCR with all tissues that have been miss-stored.
all the best
fred
Hello Dr. Pranali Nitin Shah
After death, RNA is degraded by ribonucleases already present in the cell and/or originating from bacteria or other environmental contamination. Also, there are physical and other unspecific chemical factors that may add to this effect.
So, you will get nothing.
Attached below are two articles which you may want to refer.
Article Profiling of RNA Degradation for Estimation of Post Morterm Interval
Article A microarray study of post-mortem mRNA degradation in mouse ...
Regards,
Malcolm Nobre
Here you go:
Article Ancient RNA from Late Pleistocene permafrost and historical ...
Article Ancient microRNA profiles of a 14,300-year-old canid samples...
https://academic.oup.com/mbe/article/34/4/793/2741258
Most of the research will be on small RNAs, as these are much more stable molecules than long RNAs.
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