Fellow Researchers

I was using the E.Z.N.A kit to isolate RNA and somehow skipped the step where I was supposed to spin the empty column for 2 minutes to make sure that residual Ethanol was removed. Since the kit is depleted and I don't have anything else to work with, I am tempted to proceed to the PCR test.

Has anyone done that before? Is there really enough ethanol to inhibit the PCR test?

Regards,

Alison