Hi everyone,

We have been trying to do single cell sequencing on mouse pancreas. We digest the pancreas using a kit from Miltenyi, and neutralize the digestion mixture with FBS containing media (in ~15-18 minutes). We pass this through a 100um cell strainer and centrifuge it at 4C to get a cell pellet. We stain the cells in 1% BSA containing PBS (Ca/Mg free) with EpCAM and CD133 so that we can sort for acinar and ductal cells via FACS. However, after sorting when we look at the cells on the scope, we see a lot of tiny, perfectly round, shiny structures, which almost look like cells but we are not sure if they are indeed acinar cells (since they are very heterogenous in size) or something else. I was wondering if anyone has had this experience after sorting the pancreatic cells? Greatly appreciate any inputs!