It looks like the MC3T3 may produce as high, or higher ALP, without ascorbic acid, and this seems counter intuitive. Has anyone else seen this, or noticed it in the literature? Every study I have read used ascorbic acid, so I haven't found anything published on this yet. If MC's produce ALP then is the addition of ascorbic really stimulating ALP production from differentiation?
Yes, if you treat MC3T3 with Ascorbic acid, you can increase the Alp expression much further. Anyway, be aware that some medium like alpha-MEM does already contain 50 ug/ml of Ascorbic acid.
Alkalen phosphatase is an enzyme that found in the most cells but have different tasks regarding to specific tissues. So it is found in MC3T3-E1 preosteoblast cells. Culturing it with ascorbic acid supplemented medium the ALP synthesis is triggered and increased in the cell cytoplasm day by day and reached maximum in general at day 14-21 in differentiation process.
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