14 Questions 9 Answers 0 Followers
Questions related from Michael Pujari-Palmer
Does anyone know of, or use, commercially produced surfaces for contact measurements (control material)? As an example, is there a producer of PTFE discs with highly controlled surface roughness...
10 April 2020 9,019 5 View
Is there a commercial source of well defined (i.e. known, reproducible surface roughness, flatness, etc.) "standard" materials that you can purchase? A perfect example would be a vendor that sells...
23 January 2020 4,402 2 View
Can anyone suggest a good reference for using EBSD to detect different phases of calcium phosphate? I have only found 2 papers, however they both reported that the crystal size of hydroxyapatite...
05 October 2017 3,289 4 View
Greetings, we would like to seed osteoblasts onto calcium phosphate scaffolds. We want to compare how much new mineral is formed on the scaffold depending on the composition. Can you suggest any...
07 July 2016 4,689 7 View
We are trying to see how the amount of ions such as Calcium, or Zinc change as 2-5 powders are mixed, dissolved and reacted. It is a thick, viscous mixture and we cannot use optical measurements...
22 June 2015 3,674 1 View
It looks like the MC3T3 may produce as high, or higher ALP, without ascorbic acid, and this seems counter intuitive. Has anyone else seen this, or noticed it in the literature? Every study I have...
23 February 2015 5,043 2 View
Greetings,I have fixed MC3T3 cultures and incubated with 40mM alizarin red (pH 4.2) for 12 minutes. When rinsed with water, three times for 5 minutes each, the final wash still remains darkly...
07 October 2014 2,722 2 View
Our group is investigating differences in the respiratory burst based on what type of biomaterial/surface the cells are attaching to. I am trying to understand what differences cause the...
25 December 2013 6,147 0 View
I am trying to determine whether the strength of the respiratory burst directly correlates with "how activated" the phagocyte becomes when it comes into contact with a surface/biomaterial. Can...
23 October 2013 8,245 8 View
I am currently soaking surfaces of different sized nanopores with a drug. I want to differentiate the contribution the pore has to drug release from the uncontrolled release from surface adhesion....
30 August 2013 7,100 0 View
When the Dextran 450-650k MW separates the neutrophils from RBC, after centrifuging the upper layer the neutrophils don't pellet at 250g. Any suggestions on modifications/improvements? Starting...
13 August 2013 413 17 View
I am culturing primaries and just wanted a guideline of what others have seen. Most papers only include biomaterial induced activity.
20 June 2013 9,541 0 View
Most articles don't publish the exact percentage of serum. Can anyone direct me towards a reference for any of the following lines: MD-MBA's, B16F10/F1, LLC, PC3?
16 April 2013 6,856 1 View
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16 April 2013 1,799 2 View
