How have professors been able to transition complex ideas and concepts to a virtual environment?

As COVID-19 has forced academia to react instantly to the dynamics of teaching and learning, I am curious to find out how you have been able to transfer complex ideas that you would normally teach...

17 May 2020 8,254 16 View

Can I activate a mixed primary (mouse) glial culture, and then shake microglia off?

Our lab usually uses the shaking method to isolate primary mouse microglia from mixed glial cultures. Till now, I have been collecting microglia, plating them in isolation, and then activating...

17 November 2019 2,048 1 View

What is the best molecular marker to separate healthy and pathological astrocytes?

I have quantified astrocytes with GFAP in a series of westerns. I would like to know if the lower level of GFAP I am seeing is because it is non-reactive due to a healthy environment or...

06 September 2017 5,783 1 View

My error correction term/speed of adjustment be -1.60529.?

I have performed VECM test with LLB and CPS as my independent variable and GDP as dependent variable. Can i write that In the absence of changes in LLB and CPS deviation of the model from long run...

18 August 2017 684 1 View

Do you have experience or protocols for cryofixation and sectioning of fetal/embryonic pigs?

14 December 2016 2,639 4 View

I have a mixed-design (2: condition X 2: pre-post) but due to a problem with data collection am unable to match pre-test with post-test data. Help?

I am in a bit of a bind with a project evaluating a classroom-based intervention. The design was supposed to be a mixed-design whereby I collected pre-post data from willing students linking...

05 December 2016 938 4 View

Hi, does anyone have an idea on how to purify a molecule with free thiol function without risking its oxidation?

I am interested in getting pure molecules with free thiol but the problem is that during the synthesis I always get a mixture of free thiols and disulphide bridge. After the purification on...

24 May 2016 1,699 1 View

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

14 February 2016 5,014 3 View

How long should I fix in Bouins for Masson's Trichrome?

02 August 2015 3,718 11 View

Processing for Cadaver Skin

25 August 2014 7,578 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View