I'm working on a T3/rT3 SPE extraction using an anion exchange SPE column. I have stumbled across DCM as a wash step in the literature, but if there is a non-halogenated alternative I'm not aware of, I could sure use the ideas. Thanks in advance!
Hi,
just check the elutropic series and choose/try a solvent with similar polarity. SPE is all about trying!! :)
Good luck
I guess he is talking about reverse-T3 as he stated in the question!
I´m very sorry that I was not aware of ur recently published work but I downloaded it yet.
However, Edwin wants to change the published solvent...so he has to "try" another one (and I would try one with similar polarity). Thats what I meant.
Aurea, I am extracting triiodotyronine (T3) and its isomer reverse triiodothyronine (rT3) from human serum. Currently, I have a quick conditioning step with water & method, sample load, a ammonium acetate buffer wash (10mM pH 9), 100% MeOH, and finally a 2% formic acid in DCM wash before elution.
Thomas, your suggestion with regards to polarity completely rang a bell! I decided to try diisopropyl ether, isopropanol, ethyl acetate, and 2-methyl THF. DIPE and EtOAc show the most promise thus far. Thank you for the direction!
Mhm....you never stop learning. I will definitely consider your approach from now on.
Thanx for this excursion Aurea. BR
Thank you Aurea. I will definitely take this into account in searching for other alternatives. I am by no means done with the experiments and this helps a ton! I'll be sure to read on this in your paper. Thanks again to you both.
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