I'm investigating antibodies that potentially bind circulating tumour cells. Some of the antibodies have been conjugated to the Fc region of rabbit IgG, and I suspect that the rFc can bind Fc receptors on MNC isolated from blood
If you suspect that you have problems due to binding of rFc to Fc receptors I would add polyclonal IgG (rabbit or human) to my detecton antibodies so that the added antibodies could compete for the binding and thus reduce binding of my specific antibodies. This should reduce the binding to the cells but at the same time you increase the amount of IgG. If the Fc interaction causes cell activation the increased amounts of IgG may increase the activation. If you purify your tumour cells from blood, you probably have plasma from your samples. 1 ul human plasma will give you approximately 5 ug IgG which most likely is considerably more than the amount of rFc
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