Quantifying the effect of transgene protein expression is often an arduous and multistep process if one relies primarily on protein quantification technologies such as ELISA or any of the MAS-SPEC instruments. I'm exploring new ways of measuring protein level effects as they're expressed in the phenotype and how best to capture this for a high throughput system, preferably in a field setting.
Chemometrics. You essentially use a field spec to measure a target, (meat, drugs, plants, fuel, soil, whatever) then you compare your physical measures. Programs like, Unscrambler, find the location on the spectrum that corresponds to the variation in your target variable or variables. Chemometrics is used largely in manufacturing to quickly test products as they come off a production line.
Dear Jan, hyperspectral remote sensing is definitely possible for detection of physiological changes in plants. However, some specific detectable change in spectroscopy must be identified. For example I have attached my work where the fluorescence property of green plant has been detected through atmospheric oxygen absorption band. You may proceed in a similar way and find out which spectroscopic property occurs due to transgenes. Using spectroradiometer, such as field spec : procure the uv-vis-NIR spectra of transgenic plant (both in situ and at pigment level) and compare with that of normal species.
We've had some luck discriminating between genotypes in Aspen using PLS discriminant analysis techniques on spectra derived from airborne imaging spectrometer data. You can also get at biochemical constituents of foliage using full-spectrum (field or table-top) spectroscopy. I would believe you could combine the two to get at your question.
- Badges
- Science topic
- Similar topics
- Geoscience
- Atmospheric Sciences
- Atmosphere