I have never used this program. I normally use the DNASTARLasergene set of programs, specially the SeqBuilder one. I really love this one. You can easily make virtual construct and check in advance if all your prediction are right before (e.g. that everything is in the same reading frame or the translation changes when mutating specific nucleotides..) before starting with your cloning or site directed mutagenesis.

Seqbuilder costs like £500 for a single academic licence. Haven't used vectorbuilder before, but it looks like the company is selling the plasmids that you design from the building blocks that they have available (not sure if you can use it for sequences they don't have already available).

If you are looking fro a good free software for in silico cloning purposes, I recommend ApE plasmid editor http://biologylabs.utah.edu/jorgensen/wayned/ape/

If you are asking if anyone has experience ordering from the company behind it (Cyagen), then I've not had the opportunity but I have used similar companies before and it can be much more time and money efficient than doing it yourself.

http://biologylabs.utah.edu/jorgensen/wayned/ape/

Sorry was wanting to know if anyone has used this company to make a vector. Its through Cyagen. The design we have under control but wondered with complicated vectors, if it was cost effective to have someone else construct. Thanks for your input.

Hi Trisha and all,

I am actually the Managing Director of VectorBuilder Europe and I want to inform everyone that we are no longer apart of Cyagen and have officially split off into our own company earlier this year. Petra is right that we use our extensive database of components to help build these vectors for you, both complex and simple. I know that I may come of biased (I just saw this thread randomly when trying to connect with a past collaborator) so I will tell you that if you want to try us we can build you a basic vector to close to no cost (just shipping) or give you 30-40% off of your first order if it is more complex. The site and company are now really starting to take off and as a former cloning guru for the Genetics Department at UGA I can tell you we are unlike any other company in terms of our customer service. Case and point, I now live and work in Germany and am still getting back to my clients today at 11pm at night as I enforce a strict 24hr response rate to all of my employees as I used to hate dealing with companies back when I was in academia.

Contact us and mention my name and I will make sure to get you a very nice discount on your first order to try us out. Also, keep an eye out for our huge new ad campaign in a bunch of Cell journals over the next couple months.

Cheers

I am using their services. It is really cool that from designing to ordering, it takes just minutes. It is both time and money efficient. Kudos to vector builder to create such an awesome website and functionalities! Really helpful!

I used vector builder to design and order AAV particles last year. It was my first time to use AAV. The website and customer/technical support were both helpful in designing the vectors, which worked very well.