If you mean Malondialdehyde , the answer is Yes.

We include it when we do oxylipins profiling.

Q1= 70.9,

Q3 = 41.1 (first transition)

Q3: 42.3 (2nd transition)

We measure it in negative mode using Qtrap 5500.

C18 column, reverse phase.

Please look at the following below links which may help you in your analysis:

https://www.caymanchem.com/pdfs/13987.pdf

https://www.ncbi.nlm.nih.gov/pubmed/11746912

Thanks

Orwa Barakat yes I mean Malondialdehyde many thanks for your answer

Isam Eldin Hussein Elgailani Thank you but I meant something different, i meant Malondialdehyde assay because i want to know about which column phase most used in LC-MS/MS .