Has anyone ever done plasma MDA using LC-MS / MS ??

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Orwa Barakat

If you mean Malondialdehyde , the answer is Yes.

We include it when we do oxylipins profiling.

Q1= 70.9,

Q3 = 41.1 (first transition)

Q3: 42.3 (2nd transition)

We measure it in negative mode using Qtrap 5500.

C18 column, reverse phase.

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.caymanchem.com/pdfs/13987.pdf

https://www.ncbi.nlm.nih.gov/pubmed/11746912

Thanks

Behnoush Nasr Zanjani

Orwa Barakat yes I mean Malondialdehyde many thanks for your answer

Isam Eldin Hussein Elgailani Thank you but I meant something different, i meant Malondialdehyde assay because i want to know about which column phase most used in LC-MS/MS .