Possible problem for showing 0.2 sun intensity instead of 1 sun intensity in AAA class Newport solar simulator?

We have a AAA class Newport solar simulator. We changed the Xenon Arc Lamp recently (XBO450W, Make:OSRAM) as the lifetime got over. However, the intensity is coming to be only 0.2 sun instead of 1...

14 February 2021 9,998 3 View

What might be the maximum time of immersion required for the sea water ageing of glass fiber reinforced polyester composites?

I am investigating the mechanical and physical property changes in the seawater aged GFRP. I am requiring a suggestion of to what maximum number of days should I age it. Is there a particular time...

30 January 2020 570 4 View

What is the long term impact of Zero Liquid discharge (ZLD) on overall industry??

As per Indian pollution regulators body all the distillery shall be awarded consent only if they are having ZLD scheme for treatment of waste water by the effect of regulation norms in india few...

27 April 2019 4,121 12 View

Very large difference between FEM and XFEM result, Why ?

I am using ANSYS APDL language. I am getting very large difference between FEM and XFEM result of SIF Calculation. I am solving an edge crack problem. plate dimension (20mm*10mm: Height*Width)...

28 December 2018 6,865 5 View

I have time series data of 6 variables integrated with order more than 1. Should I use Johansen or E Granger test to check co-integration?

I have learned that E. Granger test is better when there are 2 variables. But in my case there are 6 of them. Johansen Test assumes that variables are either stationary or become stationary...

30 December 2017 8,856 4 View

How to perform the Recovery in different strength of tables/Capsules by HPLC during assay and related substance test?

How to perform the Recovery in different strength of tables/Capsules by HPLC during assay and related substance test?

20 November 2017 127 3 View

Is it necessary that the activation energy of higher temperature peak should be higher than that of the lower temperature peaks of TL glow curves?

the activation energy of a thermoluminescence glow curve

13 July 2017 1,989 8 View

NDMaterials use in OpenSees

Hello Everyone! I am simulating the centrifuge test for foundation having gravel drains and pile in the liquefied soil. I started with PDMY02 soil model and everything seems fine except it is...

07 June 2017 4,419 1 View

How can I configure a network based on VoIP using OPNET modeler 14.5?

how can i configure a network based on voip using opnet modeler 14.5 ??

06 May 2015 464 1 View

Why does the nyquist plot starts from 2 Quadrant at high frequency and end at 4 quadrant at low frequency?

16 April 2015 9,228 9 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View