Has anyone attempted to measure the production of thyroxine (t4) or triiodothyronine (t3) from cell culture effluent?

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Danielle Dillon

Hi Andrew!

Arbor Assays makes T3 and T4 kits that specifically mention using tissue culture media samples.

http://www.arborassays.com/product/thyroxine-t4-enzyme-immunoassay-kit/

http://www.arborassays.com/product/triiodothyronine-t3-eia-kit/

I would definitely run through your own validations to make sure that the kit is acting as expected, but these kits might be worth a shot! Also, Arbor Assays is a great company to work with.

Good luck!

Danielle

Andrew Riley

Hi Danielle

cheers for getting in touch!

These look great, I'll certainly look into using them.

Andy

Peter H.M. Klaren

Hello Andrew,

We have measured the release of T4 from static incubations of fish' thyroid tissue, and measuring total T4 concentrations in the incubation medium with a commercial ELISA. As Danielle says: validation is important. In this case we prepared standards in the same medium (matrix) as the final medium the samples were collected in.

I think this should work for superfusion experiments as well.

Cheers,

Peter

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