Doubt....When we acclimatized an invitro hairy root producing plant to field conditions..,will the next generation from it may produce hairy roots or not?What are the genetic variations and morphological variations that are possible in it??
of curse they will produce roots because root formation is not epigenetic character. plants never get lost their root formation either in vitro or in field. It may be possible that some variation may exist.
plants will show hairy roots in vitro but not in soil in subsequent generations because hairy root trait is induced by the ingredients of medium
hairy root is induced by the bacteria Agrobacterium rhizogene its Ri plasmid is responsible for the hairy root development in the artificial medium, there are so many other microorganisms present in the soil which might affect its growth.
I would like to know perfect result of hairy root will grow or not in soil.....!!!!!
Hairy root culture means, culturing of hairy roots alone in artificial growth medium, before that the explant could be infected by Agrobacterium rhizogenesis, this bacteria will induce the formation of hairy roots in explants. The hairy root culture to be done only in lab condition.
@ Mini. Please clarify your question. Did u induce hairy roots in an intact in vitro plant or regenerated a plant from hairy root? If you have regenerated a plant from hairy root, the entire plant is transgenic and the Ri TDNA will be transferred to the next generation and the subsequent generations will produce hairy roots in field conditions also. In case if you have induced hairy roots in a normal plant, it is a chimeric plant and only the root (hairy root) parts will contain the Ri TDNA and the seeds produced in that plant will not carry any rol genes. so no question of hairy root trait in subsequent generations.
We have noticed that hairy root producing chimeric cotton plants would produce massive amount of root biomass in greenhouse conditions.
Hello Mini MS,
whatever you are using hairy roots regenerated plants or composite plants, the rol genes will start inserted so they will always show the hairy roots phenotype and characteristics as these rol genes became part of the plant genome. If you would, You can read my book chapter for all questions about hairy roots and its applications and charesteristics at molecular and morphological levels.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology