Q..A researcher desire to clone a gene (1kb) of a microorganism.Its genomesize is 1.5 * 104 kb.The average size of its library fragment is 5 kb.
A)What is the ratio of genome size of microorganism is relative to the average size of the fragment in the gene library?
a.none of above
b.3000
c.45000
d.5000
2nd part of question
B)The genomic library was created in vectors that were transformed into bacterial cells, if there is a 95% probability of the transformation, how many recombinant bacterial colonies will have to be screened to find this particular gene?
a.9000
b.10000
c.7000
d.8000
A) ans b 1.5*10^4/5 genome size/fragment length
B) there is a formula for efficientcy and probability calculation. It comes like log(1-P%)/(something which i forgot...) The formula is in Primrose book page no.approx 24. check out.. you will get it.. if i remembe ri will update...
all the best...
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