How to convert oil compound into powder for SEM?

Hello, I'm looking for a method to convert my compound into powder for SEM sample. I tried freeze drying, but the compound does not dry. Please let me know if anybody faced this issue and or...

06 July 2019 478 10 View

Does not adding PMSF and Benzamindine to my cell free extract degrade my protein of interest?

Protein Purification

08 September 2016 2,590 5 View

My protein got precipitated during overnight dialysis. Is 150mM NaCl too less for Dialysis buffer of 500ml? And i used 20mM Tris and 10% glycerol.

My protein got precipitated during overnight dialysis. Is 150mM NaCl too less for Dialysis buffer of 500ml? And i used 20mM Tris and 10% glycerol.

05 June 2016 4,982 12 View

What is the role of glycerol in preparation of competent cells of M. smegmatis?

Recombination, Plasmids, Electroporation

04 May 2016 5,658 6 View

What is the role of Acetate in TAE buffer?

Gel Electrophoresis.

04 May 2016 9,563 4 View

How to decide the degree of polarity of a buffer?

Buffer systems, Dielectric constants, pH, Isoelectric point

01 January 1970 854 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View