Dear peers and esteemed scientists,

I am running into several issues regarding growing and maintaining Xylella fastidiosa cultures. We are using PD3 media and storing the cultures in 30 % glycerol. For checking whether the colonies are of Xylella fastidiosa we are using HL5F/HL6R and holCF/R primer combinations. But lately, I observed less infection when used in infection assays on plants as well as some colonies growing with 48hrs (unlike XF also appears pinkish) and some are taking 3 -4 weeks (white opaque). I did sequence the colonies and all of them have the same sequence that matches ~98% with Xylella fastidiosa subsp. fastidiosa ATCC 35879.

Question- What are the best methods to check and confirm XF cultures?

What are the best media recipes apart from PD3 and PD2?