Hello Esteemed Researchers
I have a question and I was hoping the experts in the field could guide me. So I have never worked with Shoot Apical Meristem (SAM) and am really curious to learn more and more about it in wheat. However, I do not know how to identify its location in a grown wheat plant. I tried searching articles which researched on SAM of rice and other monocots but the method of sampling or its location has not been stated.
I would really appreciate it if someone could advise me on this as well as explain to me thoroughly on how to identify the SAM region and what would be the best procedures to sample it.
Thank you so much in advance. Any form of guidance will be fully appreciated.
With gratitude
Dee
Dear, Depika Prasad!
Typically, apical meristems are located at the top of the shoots (main and lateral). Such an arrangement of meristems is determined already in the initial phases of ontogenesis.
Perhaps, the following articles will help you by SAM in wheat:
Na-Sheng Lin and W. G. Langenberg. Distribution of Barley Stripe Mosaic Virus Protein in Infected Wheat Root and Shoot Tips
Ahmad, A., Zhong, H., Wang, W. et al. Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.)
Wang PJ., Charles A. (1991) Micropropagation Through Meristem Culture. In: Bajaj Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg
Perhaps, the following article link can help you:
Chapter Primary, secondary and tertiary meristems
Shoot apical meristem. Shoot apical meristem is the region in the growing shoot containing meristematic cells. The shoot apical meristem contains multipotent stem cells and produces primordia that develop into all the above ground organs of a plant.
Function
The apical meristem, also known as the “growing tip,” is an undifferentiated meristematic tissue found in the buds and growing tips of roots in plants. Its main function is to trigger the growth of new cells in young seedlings at the tips of roots and shoots and forming buds.
There are two types of apical meristem tissue: shoot apical meristem (SAM), which gives rise to organs like the leaves and flowers, and root apical meristem (RAM), which provides the meristematic cells for future root growth.
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