How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Is there some effective transfection reagents or methods for transfecting plasmids to ExpiCHO-S cells?

Dear all, We want to transfect pCDH plasmid or other plasmids to suspension ExpiCHO-S cells and express our target proteins, and we used the transfection reagents PEI. Unfortunately, there...

26 November 2020 8,323 3 View

No title

The cell I used is 293T.I want to it can grow on the biocompatible materials,like agarose or alginate gels.But the shape of the cells is not good.In the T25 flask you will find the cell like...

26 November 2020 1,009 2 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

25 November 2020 858 2 View

What are advantages of NELF model?

I am trying to simulate permeation of gases in polymers. For this reson normally i use the clasic models like dual sorption model but newly i foun dthe non equilibrium lattice fluid model. It...

03 February 2021 1,203 3 View

What is the role of IQAC to improve the grading of an institution?

IQAC provides all information about institution and its various activities to NAAC, Bangluru, India in order to get good grading. In this light what is the role of IQAC??

03 February 2021 6,916 1 View

IET Digital Library Access?

Hello to everyone, I do not have access to the IET Digital Library at the institution where I work. Can those who have access to this library send me the publication whose DOI is given below?...

13 January 2021 9,385 2 View

Are virtual conferences close to the results of classic conferences?

What is the approach ratio

30 December 2020 4,701 9 View

To whom to complain when a journal had accepted a paper for publication and after that the paper has been never published?

Together with my colleagues, I sent the paper to the Int. J. of Commercial Vehicles. The paper was accepted to be published on November 14, 2020. Issue (2) was published but there was not my...

30 December 2020 5,671 1 View

Can someone recommend me a classic book of liquid crystals?

I am currently into liquid crystals and have no related background. Can someone tell me which book is the most instructive one to learn basics of liquid crystals? I am more interested in different...

22 November 2020 4,362 6 View

How important are human resources in your institution? Do you think that in the current conditions it still occupies a leading place?

How important are human resources in your institution? Do you think that in the current conditions it still occupies a leading place?

09 November 2020 8,158 99 View

An author's institutional affiliation was different at the time of the study and while writing up, which one should be provided for publication?

All the researchers of a study have moved to a different institution. We conducted the research while we were at the old institution. Now, as we write-up the manuscript for journal publication,...

08 November 2020 285 3 View

What are the most challenging tasks in leading a higher-educational institution?

I would like to hear from your experience regarding the question above in leading whether a faculty or the whole institution, and how you face those challenges. My purpose is to learn new...

06 November 2020 958 6 View

Can I find a record of all my RG Achievements?

Hello mates, I'm just curious if there is a kind of RG archive, where I can find records of all my achievements, like "Your book reached 500 reads" or "With 120 new reads, your research items were...

18 October 2020 2,348 8 View