Does the technique of measurement impact the final Kd of enzyme towards its substrate?

I am trying to understand if, two different ways of performing enzyme binding kinetics may impact the final Kd values and if yes to what extent for slow and fast reactions. For more context...

02 March 2019 8,978 5 View

Is UV280 Ok for normalizing amount of protien for western?

I need to compare level of expression of a protein in E.coli cell lysates. In order to do so we want of take equal amount of protein from cell lysates. But I have too many samples to be processed...

02 March 2018 9,941 5 View

Problem with beta gal expression in E.coli K12 MG1655 cells??

I have cloned the beta galactosidase gene into a pQE-tagzyme based vector. The expression system in IPTG inducible with a T5 based promoter. Hence the promoter should be recognized by the host...

31 December 2017 1,530 3 View

Usage of high concentrations of tween 20 in western blot?

I am using IgY fractions for detecting bacterial proteins. The antibodies are verified to detect the purified protein of interest. When i use the antibodies in combination with cell lysate,...

03 April 2017 2,669 8 View

Designing primers for Primers extension assay?

I am trying to optimize a primers extension assay with 2-3 kb RNA fragments to study RNA modifications (methylation and pseudouridylation). I was wondering if anyone has an idea about the minimum...

03 April 2016 4,750 2 View

What is the use of the E. coli RRI strain?

04 May 2015 4,622 4 View

Can anyone help with handling and comparing mass spectroscopy data?

I am planning to carry out MS for a protein sample isolated after a pull down experiment with bacterial cell lysate. I expect to get a complex of proteins against the bait protein, which can be...

05 June 2014 8,963 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View