8 Questions 33 Answers 0 Followers
Questions related from Himanshu Sharma
I am trying to understand if, two different ways of performing enzyme binding kinetics may impact the final Kd values and if yes to what extent for slow and fast reactions. For more context...
03 March 2019 9,129 5 View
I need to compare level of expression of a protein in E.coli cell lysates. In order to do so we want of take equal amount of protein from cell lysates. But I have too many samples to be processed...
03 March 2018 10,053 5 View
I have cloned the beta galactosidase gene into a pQE-tagzyme based vector. The expression system in IPTG inducible with a T5 based promoter. Hence the promoter should be recognized by the host...
01 January 2018 1,698 3 View
Hi, Any suggestions for a good GUI based package to visualize MS/MS data present in excel files(*.xls or *.xlsx formats). I do not have the raw files to inspect hence the a tool that can support...
07 July 2017 1,274 1 View
I am using IgY fractions for detecting bacterial proteins. The antibodies are verified to detect the purified protein of interest. When i use the antibodies in combination with cell lysate,...
04 April 2017 2,850 8 View
I am trying to optimize a primers extension assay with 2-3 kb RNA fragments to study RNA modifications (methylation and pseudouridylation). I was wondering if anyone has an idea about the minimum...
04 April 2016 4,862 2 View
Recently i came across E. coli RRI strain, its being usually used for plasmid preps.It has carries a recA gene that has been introduced in the parent strain to create RRI. I was suspecting there...
05 May 2015 4,726 4 View
I am planning to carry out MS for a protein sample isolated after a pull down experiment with bacterial cell lysate. I expect to get a complex of proteins against the bait protein, which can be...
06 June 2014 9,070 1 View
