Is it possible to do a one to one substitution of Glyoxal for Formaldehyde when you are making FAA to fix plant tissue?
I am having minimal luck using Qiagen Stool and Plant DNeasy kits for extraction from herbarium specimens that are 20-60 years old. I use a bead beater with MP Bio Matrix A to break up my samples....
02 March 2018 4,022 3 View
My lab mates and I unable to conduct an SH test in RAxML on CIPRES. We have used a .phy file for our input, deselected "Configure Bootstrapping," supplied our best tree in a .tre file from our...
09 October 2016 2,037 1 View
Between CTAB and QIAGEN DNeasy Mini Kit extraction methods which will produce the cleanest and largest amount of DNA for Araliaceae? I am specifically working with leaf material from Polyscias...
02 March 2015 5,873 7 View
Aloha, The voucher that were collected during this trip do not have any label data associated with them. Please provide that information ASAP. Also are you planning on keeping the vouchers at...
01 January 1970 2,594 0 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View
The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...
14 July 2024 8,771 4 View