Getting pulse on oscilloscope by resistive pulse sensing method, I am trying to measure the pulses directly, but oscilloscope measurement is so noisy?

22 June 2020 1 1K Report

I made a setup for coulter counter/resistive pulse sensing following the following link " https://www.teachengineering.org/activities/view/duk_retcoulter_act1".

Followings are the steps for the setup:

I cut off the bottom of a plastic beverage bottle about 5 cm from the bottom.

I made two small holes 4-5 cm away from each other near the cut end of the bottle.

Then I attached a metal rod to a ring stand using a clamp holder.

I used 9V battery.

Two 30cm insulated wire.

I stripped the ends of the wire.

Attach the battery, 1kΩ resistor, and wires. connections is secured with electrical tape.

I placed one wire in the bottle and the other in the beaker beneath the bottle acting as a reservoir.

For making Solution

Filled a 600 ml beaker with 500 ml water.

Mixed 0.3 g NaCl into water.

Add 2-3 small drops of liquid soap.

Stir.

I used small copper sheet for making electrodes. I also make electrodes rings from copper wires. I used 1k resistor as mention in the link above and I attached oscilloscope probe across the resistor. I am using RIGOL oscilloscope MSO5354. But the problem is with the reading on oscilloscope, it is too noisy. I am getting to many fluctuation in the reading. How should I remove the noise? Is it possible to get reading directly with oscilloscope or I have to use amplifier?

Any answer or any help will be appreciated.

Abid Hussain

I changed my setup and I get pulse on oscilloscope. I 3D print a cylindrical tube shape object with 10 mm hole (channel) depth and 2 mm diameter, shown in the picture. I put the tube inside beaker and placed two stainless steel electrodes, one connected with negative terminal and the other one with positive terminal of a battery. I also attached a resistor in series with positive terminal of battery and electrode. I used 0.5% saline solution as an electrolyte. Then I drop a plastic bead of 0.6 mm, and I got a pulse on oscilloscope. I made the setup big intentionally. My aim was to produce pulses by dropping beads that can be seen on oscilloscope without any use of amplifier.

Some important note:

1. Use a battery power supply, which is less noisy compared to any other like regulators.

2. Use short probe leads to avoid ground looping effect, long probes will create huge noise.

3. Oscilloscope settings are import.

4. Attach a Digital voltmeter across sense resistor. If you are getting reading on digital voltmeter and you are not able to see any pulse on oscilloscope change horizontal and vertical setting.

5. Remove Digital multi-meter once you finished your test. Because it will add too much noise in your signal.

6. Your pulse will be in milliseconds and amplitude in mVs.

This setup will be a good small demo for educational purpose, where you don't need any amplifier or any other device except a cheap USB oscilloscope from Chinese vendor and few 3D printed parts. You can also use a bottle if you do not have 3D printer available.

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