Genetic sequence (nucleotide or protein) placement (GenBank)

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

How to predict Shine-Dalgarno sequence ?

Hello, I am Master student under molecular biology. I want to find the Shine-Dalgarno sequence after performing a BLAST search in TargetRNA2. Could you please suggest a web tool to predict the...

07 July 2024 9,598 1 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Livro DNA Sequencing Protocols de Colin A Graham ?

Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.

11 June 2024 4,042 0 View

Abhijeet Singh

Please ask a question in a way someone can answer. It would also be nice if you can put some time and effort in phrasing the question.

Sobirjon Jumamurodov

Hello. Sequencing to assess the genotype of the bacterial microflora in the upper respiratory tract in COVID-19 patients. Please help to post the sequencing data to NCBI. Thank you.

https://submit.ncbi.nlm.nih.gov/

There are also instructions about what and how to submit.

Thank you