General number-average degree of polymerization equation for bulk free polymerization in Trommsdorff effect?

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Marker gene metagenome sequencing?

The source of classification bias in marker gene metagenome sequencing? a variability in the taxonomy classification of microbial communities when using different primer pairs (e.g. for 16S rDNA)...

29 June 2024 1,048 1 View

fluorescent imaging without fixatives?

How to store SDS-PAGE for fluorescent imaging without fixatives? I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of...

29 June 2024 8,720 1 View

Is a 5` phosphate needed for homologous recombination?

Is a 5` phosphate needed for homologous recombination? I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods...

06 June 2024 7,065 1 View

Diluting an enzyme before an experiment?

What is the reason or importance of diluting an enzyme before an experiment? I'm writing a report on enzyme activity and characterisation and i need help with understanding this: we were told to...

06 June 2024 8,714 2 View

How do you get Rid of Nonspecific Bands in PCR?

So, here is what I am dealing with: The Tm of the primers is 55 for the forward one and 56 for the reverse one. In -silico PCR produces 750 BP band, but in reality I get 3 bands. The third one...

17 March 2024 9,914 5 View

PCR nonspecific product?

I am amplifying target sequence 450 bp. I get single sharp band in the control and faint in sample with another sharp nonspecific product. why? I need to get one single band from my sample to...

17 March 2024 804 3 View

CRISPR targeting in cell line, flow or single clone?

I am doing a CRISPR targeting to knock out one intron of my gene of interest in a cancer cell line and replace that intron with GFP. I am wondering if I should pick single cell clone with possible...

17 March 2024 9,713 1 View

How convert Bacterial DNA concentration to copy numbers?

For example, If a PCR primer can detect ~100 fg of DNA, how we will calculate it to Bacterial copy number?

17 March 2024 1,816 2 View

Guide RNA for improved CRISPR-Cas9 gene editing ?

How can the design and synthesis of guide RNA molecules be optimized for improved CRISPR-Cas9 gene editing?

05 August 2023 1,375 2 View

Can it be used for large gene production (4 kbp) via CRISPR-mediated HDR?

I could not find a study that used CRISPR-mediated HDR for stable integration of large genes. I would be grateful if anyone could point me in the right direction.

05 August 2023 486 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View