Gene regulation and epigenetics

07 November 2012 1 4K Report

I have a gene sequence and want to know how its expression is regulated. whether Epigenetic modification analysis suitable? What should be my focus: CDS or promoter region or regulatory region? How can I correlate transcriptome data to epigenetic regulation?

Amit Mahendra Karole

Because dna modification alone unefficent to regulate gene expression hence i will suggest u to go for chromatin immunoprecipation this will give u an idea of epigenetic

modification associated with it and then go for rna expression study by rt pcr.

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