Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Matrix based scientific data analysis: extrapolating to matrices of sets?

Data sets, when structured, can be put in vector form (v(1)...v(n)), adding time dependency it's v(i, t) for i=1...n and t=1...T. Then we have a matrix of terms v(i, j)... Matrices are important,...

30 January 2021 9,295 6 View

SELECT IF in SPSS and its Syntax?

I am writing a piece of code in the Syntax of SPSSstatistics. The code is the following. GET DATA /TYPE=XLSX /FILE='file' /SHEET=name 'Sheet2' /CELLRANGE=full /READNAMES=on...

14 January 2021 2,463 8 View

Statistical code for indexes?

05 December 2020 664 3 View

Non-parametric correlation tests on SPSS?

I have computed multiple Kendall tau-b and Spearman rho on SPSS. For some of my instances, Spearman rho index is 1 and the Kendall tau-b is 0.988. It should be also the Kendall tau-b 1. How...

21 November 2020 9,833 3 View

Human-Machine co-operations or competition? Where are we today?

At the cross-roads of Technology for Advanced Manufacturing, involving robots and humans, and Digitised Economic Sectors, is a governance matter for Cognitive Manufacturing [1] and rules of the...

21 November 2020 7,145 20 View

LIFE in SMART CITIES: Animal data versus Human data, complementarity or not?

One observation is that in any city (those I know) you have humans, of course, but you also have many animals. Statistics of humans start with population data (for instance demographics) and...

08 November 2020 4,127 6 View

ISSR Annealing Temperature Calculator, which formula can I use?

I was wondering if I can use a formula for my ISSR primers to calculate the annealing temperature and melting temperature. Alternatively I would like to know if is possible to calculate with any...

27 October 2020 8,885 3 View

Recommended insect storage/shipping method for NGS ?

Dear all. We need to sample insects, store them upon collection and ship them internationally. Sampled will later be processed for DNA extraction and NGS sequencing. Keeping all this in mind, can...

25 October 2020 7,390 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View