Gating strategy for murine spleen neutrophils?

05 May 2020 0 9K Report

I have started doing flow experiments to count neutrophils in murine spleens.

Who can help me designing a good gating strategy for it? I'm using CD11b and Gr-1 as markers (and of course a live/death dye) now. I will introduce a Ly6G marker since it is specific for neutrophils, but is it possible to get a clear neutrophil population with the previous 2 markers?

Thank you in advance!

Giulia

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