Need to discuss related to Future Work on Biomethane from Food Waste and their Life Cycle Assessment
I'm currently working on a retrovirus. I'm having trouble detecting protein using IP-WB methods. I have the antibody-it's polyclonal, but I don't have antibodies conjugated with beads for the IP...
19 April 2022 625 7 View
I'm currently working on a BLV experiment. I tried to construct the BLV env gene using a primer that contained `a myc tag antibody`, but when I checked the protein, I was unable to find the...
29 April 2021 7,249 1 View
Hi, I transformed a PCR product containing GFP from pGFPuv without Lac promoter (change it with 50bp homologous arm for pRED/ET recombination) into Enterobacteria by electroporation. I expected to...
11 November 2020 8,852 3 View
How to differentiate Leuconostoc, Weisella, and Oenococcus. Recently we are working with LAB and found that the isolates were Cocci, Gram positive, catalase negative, non-spore forming bacterium,...
11 May 2017 8,980 4 View
Dear all, I assembled MiSeq reads using SPAdes. The sample is an endosymbiont bacteria. I found a long contig, almost 100 Kb, good coverage. At first, I thought it was part of my target bacteria...
07 May 2016 1,286 1 View
Some developing countrys' problem regarding fish preservation is employing formaldehyde to prolong the freshness of fish during catching. A high amount of formaldehyde is found in fresh fish sold...
11 October 2015 4,589 7 View
I had a problem to find simple protocol to analyze total free amino acid in fermented fish and shrimp. any suggestion? Regards
25 May 2015 9,509 6 View
I read some publication related isolation of novel actinomycetes isolates from sea cucumber but not yet find some publication related to simbiotic actinomycetes in sea cucumber that produce...
15 April 2015 5,541 2 View
Hi, I have problem when storing lactic acid bacteria employing glycerol 5% and 50%, and then problem occurs in our lab, the freezer's temperature down until -10 degree. after one month I culturing...
01 March 2015 7,688 14 View
Some problem in shrimp culture is protozoa, but I don't have idea to culture or isolate particular protozoa from shrimp gut, I wondering to get some idea to do that.
15 February 2015 9,414 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What role do microorganisms play in the cycling of matter and role of microbes in the biogeochemical cycle?
25 July 2024 3,096 2 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
What is climate resilience for food security and sustainable agriculture and how is smart irrigation in agriculture climate resilient?
22 July 2024 1,947 0 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
How can sustainable agriculture practices mitigate climate change and ensure food security and role of agriculture in environmental sustainability?
17 July 2024 1,712 12 View