What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Can someone recommend me a classic book of liquid crystals?

I am currently into liquid crystals and have no related background. Can someone tell me which book is the most instructive one to learn basics of liquid crystals? I am more interested in different...

22 November 2020 4,362 6 View

How does the influence of metal ions on the fluorescence intensity of a compound?

We found an interesting phenomenon that metal ions could not influence the fluorescence of a compound when the compound was dispersed in solvent. However, the fluorescence of the compound was...

31 October 2020 6,568 3 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Fluent how to draw a graph of coordinate changes over time?

When simulating waves, it is necessary to detect the coordinate change at a certain point of the wave surface, but there is no in fluent.

04 October 2020 1,541 1 View

How to quickly know the new research area landscape and find a research gap in this new area?

As a Ph.D. candidate who enters a new research area that differs from the research topic in the master period (e.g. shift form crop breeding to soil ecology), how to quickly know the new research...

23 September 2020 8,131 5 View

How to build epoxy model in GAFF for Lammps?

Dear all, I am a beginner in Molecular Dynamics. I build a pre-reaction epoxy model using Materials Studio and plan to perform the cross-linking procedure using Lammps. However, the msi2lmp tool...

26 August 2020 7,697 1 View

How to detect brown pigments generated in red wine aging process ?

During aging or storage, the concentration of monomeric anthocyanins decreases rapidly, while low level of polymeric anthocyanins generates, and the color of wine turns brick-red gradually. In...

17 July 2020 6,032 4 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

Testing of Antennas?

So, I came across, where antennas are being tested in a building ( a plant), where all sorts of big equipment are around and of course the ceiling. lot of multipath which can affect the...

21 January 2021 9,437 3 View

Should sampling and reconstruction be matched? How should the sampling be for machine learning algorithms?

In image reconstruction, we usually design reconstruction algorithms in such a way that we can choose the most convinent sampling pattern. However, the choice of the sampling pattern has a...

10 November 2020 8,669 1 View

Video feature extraction and reconstruction?

The traditional target detection or scene segmentation model can realize the extraction of video features, but the obtained features cannot restore the pixel information of the original video (if...

09 November 2020 1,601 6 View

What is the best way to apply non-negative matrix factorization (NMF) to a very sparse matrix?

Hello, I wish to apply the NMF algorithm to an extremely sparse matrix (~15% non zero values) with the intent to cluster the data based on the similarity of their feature matrices. Because of the...

01 November 2020 3,398 2 View

How to capture the geometry of rubble piles / collapsed structures beneath the surface via remote sensing?

Hi dear community, I am currently working on a project where I want to generate a 3D reconstructed model consisting of rubble piles (in consequence of a building collapse) via remote sensing. In...

10 September 2020 4,685 4 View

Functional analysis by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). How is this analysis done?

I would like to analyse fish gut bacteria by the Functional analysis by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). How is this analysis done?

04 September 2020 3,048 1 View

Are you interested in learning about post war reconstruction?

Third edition application is open soon... if you are an Engineer, medical doctor, teacher, manager ...etc. and interested in learning about reconstruction, and development management , this is...

06 August 2020 1,139 3 View

Steps to improve cryoEM helical reconstruction resolution using relion?

Hello all, I am trying to do helical reconstruction for my fibrils. I am at 3D classification steps where I can get the resolution (given by relion) at 3.2 A. The actual resolution is worse I...

28 July 2020 6,266 1 View

Is there any datasets to test a thresholding method against a groundtruth?

Hello, I am currently testing a thresholding method and I would like to test this method on a dataset of images with ground truth to see how good it works. I am not trying this thresholding as...

17 June 2020 751 1 View

What are some of the free sources where i can download paleo-climate data ? What things you take into considerations while processing paleo data ?

Greetings, I am planning to work with open source paleo-climate data for a thesis, so far the only source for these kind of data i know is : https://www.ncdc.noaa.gov/paleo-search/ Is there...

28 May 2020 9,084 14 View