Has anyone had weird polybrene toxicity during viral transduction?

Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...

26 January 2021 4,071 3 View

No title

02 January 2021 7,417 10 View

What could be the cause of the Glutathione peak migration (LC Dionex, Kintetex EVO C18, water phase) ?

Hello, I need a help from those of you who work on Kinetex EVO C18 with water + 0.2% TFA mobile phase (isocratic). The conditions are constant (temperatures, flow, column, conditioning time)....

16 December 2020 4,582 3 View

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

14 December 2020 7,845 19 View

Macrostudies using sysGMM?

Dear all, Could you recommend macroeconomic studies (hopefully published in good journals) that use system GMM or Arellano-Bond estimators? Thanks a lot!

10 December 2020 5,130 4 View

What do you think about the article in Scientific American that nobody knows how planes lift off and fly?

https://www.scientificamerican.com/article/no-one-can-explain-why-planes-stay-in-the-air/

07 December 2020 4,404 19 View

No title

07 December 2020 9,722 12 View

Why my Nyquist plot does not intersect the x-axis?

I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...

03 December 2020 8,471 1 View

GPower for Three-Way ANOVA analysis?

I want to explore if the amount of time (usage) spent on the Social Media IV1, with two levels , one is light and the other is heavy use, Iv2, body image with negative and positive levels, Iv3...

17 November 2020 4,405 3 View

DNA extraction from bacterial culture?

Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...

06 October 2020 6,890 8 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

Can people point me to interpretative phenomenological analysis (IPA) studies where there are only two participants?

I am in search of studies with a small n to support recent work completed in-depth with two participants regarding international students in Canada. I am not sure if framing things as two case...

23 January 2021 8,564 7 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View