Fungi species in the Cercospora genus are slow-growing fungi, known for their cause of the leaf spot disease in many crops.
Due to their slow-growing nature, culturing them is combatted and overtaken by other fast growing fungi contaminants, therefore, it is important to use specific growth medium in their laboratory culture.
Have you tried a crude homogenization of plant tissue in water followed by serial dilution? You can then use a scalpel to isolate individual colonies.
Consider adding some fungicides that your species is resistant/insensitive against to inhibit competing species (Article Fungicide resistance in Cercospora species causing Cercospor...
).
thank you Robert Adolf Brinzer for your beautiful recommendation. i will try it out in my experiment.
appreciate
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