I am trying to research on cell recovery
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
We are carrying out trial in the glasshouse where we are comparing the infiltration rate in sodic soil in two different treatments Polyacrylamide vs organic matter ( lucerne pellet). within 27...
25 November 2020 8,787 3 View
I am doing alkane oxidation using m-CPBA as oxidant in DCM. How can I remove the oxidant and its corresponding acid (m-CBA) from my reaction mixture? I also did aqueous work-up using NaHCO3 and...
13 August 2020 8,290 3 View
commonly used practices for the identification and characterization of the kind of cell in cord blood other than Flowcytometry and reverse-transcription polymerase chain reaction (RT-PCR).
08 August 2020 6,881 3 View
Agriculture is the major contributor to the national economy, livelihood and employment since long. However, despite the priority in the national policies, the growth of the agriculture sector is...
05 June 2020 1,079 2 View
Hello all, I have 60 samples of Whole Genome Sequence (BAM, VCF ) File. Now I want to do SNP analysis ( I am trying to find some positions which differ among case and Control ). I did with...
30 May 2020 980 4 View
As internet is, now-a-days, a useful tool for data collection and research, issues also go hand in hand with ethical approval and informed consent. As participants are, by default, give their...
25 May 2020 4,055 13 View
Let us consider, I collected baseline data for control and experimental group. After that i give intervention to experimental group. Later, I collected data from both group after intervention. If...
18 May 2020 6,007 7 View
Is it possible that an anticancer drug IC50 value for a particular cancerous cell line decreases on conjugation with an antibody??
22 April 2020 9,482 4 View
In Field ion microscopy (FIM), polycrystalline tungsten nanotip is (110) oriented tip. under the gas etching process, the nanotip becomes more sharp by reducing the atomic layer. During the...
14 January 2020 8,055 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...
26 February 2021 1,511 3 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View
I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...
14 February 2021 300 3 View
I understand that the ability of standard salt solutions commonly used to produce a certain relative humidity (RH) in a closed space changes somewhat with the temperature. Where do I find data...
04 February 2021 7,370 4 View
I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...
28 January 2021 3,867 1 View
Hi everyone! I am purifying a peptide from a mixture of proteins. The peptide (3 kDA) is in a solution containing a high concentration of urea and imidazole. I would like to be able to change the...
27 January 2021 4,055 4 View
A huge amount of water is evaporated to cool down and solidify the liquid metal strands coming out of bottom of CCM mould. The steam contains significant latent and sensible heat energy, besides...
26 January 2021 3,446 4 View
pH 13 Buffer solution is prepared from a strong base sodium hydroxide and potassium chloride salt. However a buffer contain a weak base or weak acid with their salts. What is the purpose of the...
26 January 2021 9,154 1 View
