For Neurobiology people

More Kishore Golla's questions See All

Which MWCO filter to use to get rid of unwanted proteins?

I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...

05 June 2024 691 1 View

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

28 March 2024 259 3 View

How many number of moles of steam required to quench one mole of carbon monoxide(g) ?

Share the reference of number of moles of steam (practically / experimentally) required to quench one mole of carbon monoxide(g) ?

20 March 2024 8,316 0 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

What is your feeling in current system of education?

Education is imparting stress to each and every individual. There is heavy competition and all are striving to reach the limit. There are plenty of things to do and there is nothing in specific....

04 January 2024 9,726 1 View

It is possible to dope Zr or any other rare earth metals in graphene oxide?

Can anyone explain the possibilities of doping Zr, Ce, or any other rare metal in graphene oxide?

02 January 2024 5,431 3 View

How to generate supporting files from CIF?

How to generate supporting files from the .CIF file for submission in the CCDC database? Kindly share any tutorial videos that could help me a lot. Thank you in advance.

27 November 2023 9,582 1 View

I have information regarding the tribal practice to combat Classical Swine fever, Can somebody from India help en route to develop some product for?

There are 6 plant materials, (which were used all along equally) by certain tribes in Jharkhand to combat African swine fever with 100% results without any targeted vaccination. I want a...

15 August 2023 9,347 0 View

How does core subjects in Engineering will become on par with computer science subjects?

Now a days, core subjects in Engineering such as electrical, electronics, chemical, mechanical and civil apart from computer science are getting least priority for option. These days everything...

23 May 2023 2,986 4 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

Does the potentiostat have to be in a fume hood when polymerising the PANI?

I will polymerise Aniline on ITO glass WE, using 1M of H2SO4 and a potentiostat. However, I am not sure if I have to do the electrochemical polymerisation inside a hood. Is it necessary? Is the...

10 July 2024 2,937 3 View

Where to source glass rings compatible with cell culture?

Hi all, I am looking glass cilinders that have an open flat bottom and top. Preferably with the inner diameter in the range of 2 cm. It's similar to the ones found on multi-electrode arrays for...

06 July 2024 5,846 1 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View

Why PDMS is not hardening ?

I use PDMS to build a microfluidic system, but it is not hardening at all, although I use TEOS as a cross-linker and also ATPES in a ratio of 1:10, and it's been 3 days at 80 degrees of 3 ml cast...

01 July 2024 604 3 View

To generate hydroxyl radicals the photcatalyst need to be in conatct with water or air pollutant?

Is direct contact of polllutant and catalyst needed? or indirect contact just like photocatalyst wrapped inside glass can also create hydroxyl radicals and elimnate pollutants near to its wrapped...

23 June 2024 3,249 5 View

Where can we get few mm diameter polished circular glass plates?

who retails few mm diameter polished circular glass plates ? thickness 0.1 to 1 mm diameter 1 to 3 mm ordinary or fused silica glass polished on both sides and the edge

16 June 2024 763 1 View

What is the materials composition of automotibles?

What share is made of steel? Plastics and glass? Aluminum? Iron?

15 June 2024 7,246 2 View

Is there way to remove apolar residue from glass in order to perform IR-MS?

I used a two-phase extraction protocol (essentially the Bligh-Dyer or Folch protocol) to fractionate C. elegans into aqueous metabolites, lipids, and protein. These worms have been fed bacteria...

11 June 2024 8,991 1 View

Replacement for acetone in dehydratization of cryo sections?

We are currently trying to replace acetate when dehyrdating ("drying") cryo sections on glass slides with a less harmful alternative. For many chemical purposes ethyle lactate is recommended - has...

10 June 2024 5,213 3 View