Dear fellows,
I am wondering about, how many bioinformatic tools are required to declare a variant as pathogenic and deleterious?
Infinite, because the question is not finite/logical. You will get a better answer if you mention the actual task you want to perform and how to do it.
NaN or 01111111110000000000000000000000 (binary representation by IEEE-754).
If its a pathogenic or likely pathogenic variant. Most probably different tools will give you the same prediction.
If its VOUS, you have to prove the pathogenicity.
1. Segragating the variant within a minimum of 10 family members.
2. Expression analysis.
3. Western blot.
4. Protein Modeling.
best wishes,
Umair
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