Plz refer the attached guidelines. It depends on the method that you use and could vary from 1-5 per dose.

I prefer to have at least 10 rats for acute effects/

Thanks & Happy New Year

8-10 rats.

Thanks. Maximum 6 rats to be taken as CPCSEA- Institutional Animals Etics Committee (IAEC).

Are you sure you need an acute study? European pharmaceutical companies have demonstrated that these studies are not needed prior to first clinical trials in humans (Robinson et al. A European pharmaceutical company initiative challenging the regulatory requirement for acute toxicity studies in pharmaceutical drug development. Regul Toxicol Pharmacol. 2008 Apr;50(3):345-52.) 

Thanks

Hi,

I was part of this initiative cited by Sebastian Hoffmann and I would agree that these studies are not need anymore. There is plenty of high-dose preclinical data being generated during early durg devolopment process that is more than sufficient.