When presenting circadian bioluminescence data (e.g., from Lumicycle recordings) for a given cell line, is it methodologically sound to average the raw or baseline-subtracted traces from three biological replicates and display a cosine fit with standard deviation? Or is it more appropriate to fit each replicate individually?
Libor Krásný
If the signal is strong (compared to background), you should get the same result irrespective of the background subtraction approach. This said, the second approach (to fit each replicate individually) is the more correct method.
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