Dear experts
We have several full test methods for evaluating phenolic compounds and I am pretty sure that folin-ciocalteu (FC) method has been applied by many of us.
I'm looking for a standard protocol that explains step by step.
In one method just mentioned fc 1:5, another is 1:10 even I saw 1:1.
For some references, the alkaline agent is 29.5%, about 7% and about 5.5%.
The inoculation time also varies from 24 hours at room temperature to 30 minutes at 40 degrees Celsius.
No protocol exists for the standard solution, only the preparation of the solutions.
The volume of the samples, the different concentrations and the other agent are also different.
The absorbence is between 745 and 780.
Can I ask you to rely on your background and experience?
Regards
Dear Reza Farzinebrahimi ,
Please find my latest publication here which may related to your research of detection of TPC.Article Evaluation of the chemical composition and nutritional poten...
For the wavelength of measurement. I think you could detect your standards curve in different wavelength and find the best wavelength with highest correlation coefficient (R square).
Best regards,
Weihao
Dear Meng
Many thanks for your kind attention.
Normally, I follow a protocol from my previous publication, but I eagerly await a standard and an applicable method.
Regards
Nataliia Savinchuk you should probably gave some experience with Folin-Ciocalteu (FC) method, right?
Hello!!! This is an article that contains a standard protocol, explains step by step.
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