Fluorescent derivatives of phenolic compounds?

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Convergence of absorbency values to determine the total phenolic content of several solvents?

I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help

17 July 2024 5,151 5 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

Asked a separate ways?

Dear all, I have a question regarding ways to separate ammonium nitrate and phosphorus pentoxide？

12 July 2024 3,164 2 View

Adam B Shapiro

Phenol itself is fluorescent.

https://omlc.org/spectra/PhotochemCAD/html/072.html

Guobing Xiang

Condensation of 2x1,3-diphenol with 1,2 di-benozic anhydride (Flourescein dye)and condensation of 2x 3-aminophenol with 1,2 di-benozic anhydride (Rhodamine dye)

Sara Darwish

@Adam B Shapiro, yes phenolic compounds are themselves fluorescent but I need to shift the fluorescence to long UV wavelength