Flt3 and NPM1 mutation in AML patients

19 February 2011 1 9K Report

I would like to look ofr Flt3 and NPMI mutation in our patients with Acute Myeloid Leukemia. I was wondering if we could use peripheral blood sample for DNA or is it necessary to have bone marrow sample. Kindly suggest

Best regards

Saqib

Didier JAMBOU

Dear Colleague,

In our lab, we determine Flt3 mutation in AML patients by classic PCR, on DNA extracted by mean of Qiamp DNA mini blood kit, either in bone marrow or in blood samples.

We don't performed NPM1 mutation determination actually in routine , and we have to validate it. But it could be the same.

Best regards

Didier J

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