Flavonoid-to-Phenol Ratios in
Methanolic Extracts of Aspilia africana:
Implications for Managing Oxidative
Stress
@Yurii Yes, both flavonoids and phenols are phenolic compounds, meaning they contain one or more hydroxyl (-OH) groups attached to an aromatic ring. They are classified under polyphenols, a broad category of plant-derived secondary metabolites known for their antioxidant properties and health benefits. The study focuses on the ratio of flavonoid to phenol in the extracts of the different parts of the plant.
Antioxidants are one of my area of interests. Your post is just an advertisement of your own paper.This paper is based on application of the wrong assay for flavonoids (see Article Determination of total flavonoid content by aluminum chlorid...
.The Folin-Ciocalteu assay measures all polyphenols including flavonoids. Thus, you can't determine the ratio of phenol/flavonoid. Make your own conclusions about the scientific value of your paper. I would advise to delete this post.
@Yurii Geletii Thank you for your contribution. However, you should know that my study focused on determining separately the concentration of flavonoids and simple phenols using an existing assay method (not proven to be incorrect) in the plant extracts and mathematically calculate the F/P ratios using a formula developed by us. If you say the method I used is wrong, which other methods do you think are correct? Please your reply will be appreciated. Thank.
I know well the chemistry behind of both these assays. The Folin-Ciocalteu assay measures all polyphenols including flavonoids. The asay for flavonoids has numerous problems. Thus, you can't determine the ratio of phenol/flavonoid using simple assays procedure. Flavonoid and polyphenol analysis are the complex
and expensive, e.g
https://metabolomics.creative-proteomics.com/
The assay of antioxidant activity is the another complex story.
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