Hi all! I am looking for help on the best fix brine shrimp (Artemia salina) in 4% PFA.
Does anyone have experience with this?
Since these shrimp have an exoskeleton, how long should I fix them for at 4 degree C?
Any literature or suggestions on how to perform this fixation would be extremely helpful! Thank you in advance!
Ahmad Al Khraisat
Prepare the PFA solution. Mix 4% PFA in a suitable buffer, such as phosphate-buffered saline (PBS), to create a suitable concentration for fixation. Place the brine shrimp in the PFA solution. Carefully transfer the brine shrimp to a container containing the PFA solution, taking care not to damage the exoskeleton. Incubate the brine shrimp in the PFA solution at 4°C. Allow the brine shrimp to incubate in the PFA solution at 4°C for a suitable period of time, such as 24-48 hours. This will allow the PFA to fix the brine shrimp and preserve the structural integrity of the exoskeleton. Rinse the brine shrimp in PBS. After the incubation period, rinse the brine shrimp in PBS to remove excess PFA. Store the brine shrimp in PBS. Transfer the rinsed brine shrimp to a container containing PBS, and store them at 4°C until they are ready to be used for further experimentation or analysis.
To fix brine shrimp (Artemia salina) in 4% paraformaldehyde (PFA), you can follow these general steps:
It is important to handle the brine shrimp carefully during the fixation process to avoid damaging the exoskeleton. You may want to refer to published literature or consult with experts in the field for more detailed protocols and recommendations for fixing brine shrimp.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Medicine
- Public Health
More Jason Jacoby's questions See All
Similar questions and discussions