How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to control tap coefficient (a_n) in microwave photonic filters?

Respected Researchers Refer to the yellow highlighted text, Eq. 6, Fig. 1 and 2 of the attached publication, Please let me know how to control filter coefficient (a_n) mentioned in Eq. 6 with...

18 January 2021 4,724 2 View

Can I publish my patents as research articles, dear colleagues?

Sometimes we have some patents that can also be published as research articles in well-known journals. Is it therefore possible to use your own patented material and publish it as a research article?

11 January 2021 8,399 8 View

What is Cox-regression analysis and Kaplan Mier Curves and how can I apply them on SPSS?

SPSS, Data-anaylsis

07 September 2020 3,298 7 View

How to solve the "zero count rate" of dynamic light scattering machine?

Hi, When I start the measurement after putting my sample in DLS machine, it shows zero count rate and didn't measure the readings. I have also attached the screenshot for your kind consideration....

17 August 2020 5,826 3 View

Anyone who is using FLAC 2D Version 8. I have version 7, before upgrading to version 8, I want to check large model with small mesh is working or not?

I need a large model say 100 x100 m with a mesh size of 0.05m. such a large model cannot be made in version 7. before upgrading to version 8, I need to confirm whether it is working in version 8...

22 July 2020 2,447 9 View

Machine Learning Method for Smaller Data set?

I would like to forecast the flashover parameters (surface resistance, inception voltage and flashover voltage) of polymeric insulators using four input features. But the size of the samples is...

07 April 2020 6,267 16 View

Which solvent is suitable for polypyrrole?

CONDUCTIVE POLYMER

19 June 2019 1,001 3 View

What are your recommendations for mounting oil of cancer cell staining , I am looking to stain microtubules and actin in slides under confocal mic ?

What are your recommendations of mounting oil for cancer cell staining , I am looking to stain microtubules and actin in slides then imaging under confocal microscope ? I am using oil...

08 April 2019 4,588 1 View

How can we analyse the security level ???

Please guide me to answer the above question. I need to know how can we measure security level???? How can I say this application or system or network is secure or under threat or targeted??? What...

17 September 2018 6,996 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View