Hello,
I want to analyse the fibersof apples. There is the AOAC 985.29 and 2009.01 for fiber analyses. Has anyone experience with the methods? Do I have inulin, oligosaccarides and resistant stark in apples?
Catherine M G C Renard
No, no inulin, no resistant starch (well, only native starch, no retrograded starch), no significant amount of oligosaccharides.
However none of the methods are really good for apple (fruit) fiber quantification as apple pectin, which is both abundant and highly methylated, is degraded during the hig temp / "high pH" steps (for a pectin pH above 5 is a high pH). Remember that all these methods were designed for cereals.
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Harasit Kumar Paul
You may go through the following link.
Article A comparison of three methods for analyzing dietary fiber in 38 foods
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